Prof. Li, chun-chun
TEL:+886-6-275-7575 ext.58135 研究室:58104 or 58114轉34
vesicular trafficking, protein transport, and cytoskeletal modulation
Cell motility is critical in diverse biological processes, including embryonic development, immune surveillance, and wound healing. Directional migration of cells, usually in response to external chemical or mechanical cues, requires complex actions in membranes trafficking and the cytoskeleton modeling that are precisely coordinated, both spatially and temporally. Although many of the individual molecular machineries have been elucidated, the mechanisms coordinating regulation of vesicular trafficking and cytoskeleton are not clear. ADP-ribosylation factor (Arf) GTPases and their regulators, Arf guanine nucleotide-exchange factors (GEFs) are likely links among individual events.
Two ~200-kDa brefeldin A-inhibited guanine nucleotide-exchange factors (GEFs) BIG1 and BIG2, like other members of the Sec7 family of Arf GEFs, accelerate the replacement of Arf-bound GDP with GTP to initiate vesicle formation for transport of cargo among cell organelles. BIG1 was seen concentrated at trans-Golgi network, where it partially overlapped BIG2, which was associated also with recycling endosomes. In addition to the Sec7 domain, BIG1 and BIG2 also possess extended N and C termini with several domains that functions remain unclear. Only a small fraction of these large molecules interacts with Arfs, suggesting multifunctional roles for BIG1 and BIG2. Mutations of BIG2 were linked to autosomal recessive periventricular heterotopia, a developmental disorder of neuronal migration leading to severe malformation of the cerebral cortex. Our previous studies in cultured cells showed that BIG1 and BIG2 are implicated in regulation of cell polarization, integrin trafficking, and actin dynamics for cell migration. However, the upstream molecules and signaling that regulate BIG1 and/or BIG2, and molecular mechanism via which BIG1 and BIG2 regulate vesicular trafficking and cell migration remain unclear. Elucidation of the detailed molecular mechanisms of BIG1 and BIG2 action, and identification of additional components of BIG1 and BIG2 functional complexes by using the techniques of cell biology, molecular cell, biochemistry and proteomics are goals of our continuing studies.
|Ph.D. Degree||Institute of Molecular Medicine, National Taiwan University, Taipei, Taiwan|
|Bachelor’s Degree||Department of Biology, National Cheng Kung University, Tainan, Taiwan|
|Aug 2013~ present||Assistant professor||Department of Life Sciences, National Cheng Kung University|
|Oct 2008~July 2013||Postdoctoral Fellow||Cardiovascular-Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, USA|
|Sep 2007~ Sep 2008||Postdoctoral Fellow||Institute of Molecular Medicine, College of Medicine, National Taiwan University|
Major Awards and Honors
|2009||Distinguished Thesis Awards, Tien Te Lee Biomedical Foundation, Taiwan.|
|2008||Graduate Student Research Award, National Taiwan University College of Medicine. Taipei, Taiwan.|
|2007||Poster Presentation Distinguished Achievement Award, The 15th Symposium on Recent Advances in Cellular and Molecular Biology, Kenting, Taiwan. The Chinese Society of Cell and Molecular Biology.|
|2003||Poster Presentation Distinguished Achievement Award, The 11th Symposium on Recent Advances in Cellular and Molecular Biology, Kenting, Taiwan. The Chinese Society of Cell and Molecular Biology|
- Li, C.-C., Lin, C.Y., Huang, P.H., and Lee, F.J.. (2003). A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1. The 11th Symposium on Recent Advances in Cellular and Molecular Biology, Kenting, Taiwan.
- Li C.-C., Chiang T.-C., and Lee F.-J. S. (2005) “ ARF-like 5 Protein (ARL5) interacts with Cytohesin-2/ARNO and modulates its function on actin remodeling” The 45th Annual Meeting for American Society for Cell Biology, San Francisco, CA, USA, Dec 10-14, 2005.
- Li, C.-C., Chiang, T.-C., Wu, T.-S., and Lee, F.-J. S. (2007) ARL4D Recruits cytohesin-2/ARNO to modulate actin remodeling. The 15th Symposium on Recent Advances in Cellular and Molecular Biology, Kenting, Taiwan. The Chinese Society of Cell and Molecular Biology.
- Li, C.-C., Chiang, T.-C., Wu, T.-S., Pacheco-Rodriguez, G., Moss, J., and Lee, F.-J. S. (2007). “ARL4D Recruits Cytohesin-2/ARNO to Modulate Actin Remodeling” The 47th Annual Meeting for American Society for Cell Biology, Washington DC, USA, Dec 1-5, 2007.
- Li, C.-C., Kuo, J.-C., Kiyama, R., Moss, J., and Vaughan, M. (2010) Effects of BIG1 and KANK1 on cell polarity and directed migration during wound healing. The 50th Annual Meeting for American Society for Cell Biology, Philadelphia, USA, Dec 11-15, 2010.
- Li, C.-C, Shen, X., Aponte, A., Shen R.-F., Billings, E.M., Moss, J and Vaughan, M. (2012) Brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 2 regulates cell migration via effects on integrin β1 cycling and actin cytoskeleton remodeling. Experimental Biology 2012, San Diego, CA, USA, April, 21-25, 2012.
- Li, C.-C, Le, K., Moss, J and Vaughan, M. (2013) Interactions between Arf guanine nucleotide-exchange factors, BIG1 and BIG2. Experimental Biology 2013, Boston, MA, USA, April 20-24, 2013.